Case Analysis Admiralty Law - 2141 Words

Admiralty Law Name Institution Course Date Admiralty Law Facts On 1 February 2012, an oil tanker known as The North Star operated and owned by Poseidon Ltd, is grounded a mile near the port of Swansea. The tanker broke up and started leaking crude oil into the sea. The leak is then washed up on the beaches surrounding Swansea. The tankers’ grounding was wholly caused by Jeremy’s negligence, a pilot employed by SHA, Swansea Harbor Authority. Due to heavy seas, salvage attempts for the vessel became difficult. As a result of this, it took The Ocean Salvation crew about four days to reach the grounded tanker. At this point, more than 10,000 tonnes of crude oil had spilled into the sea. The Ocean Salvation is operated by Seamorgan†¦show more content†¦All the guests in his hotel cancel their holidays in February and March. The losses he suffered in terms of revenue amounted to  £50,000. A local cockle angler – Huw, suffers substantial losses following the step by the UK government to enact the Emergency Restrictions A ct in 2012, which banned the harvesting and sale of cockles within the area of the oil spill. This ban lasted close to 18 months and Huw having no other alternative source of income suffered significantly. Another local resident, the owner of Fantastic Fauna Ltd – Dewi who runs boat trips to observe the harbor seals found in Swansea. Due to the oil spills, scientist observed that the seals had been permanently displaced from Swansea and had instead settled in Cardiff. Due to the oil spills and consequent closure of hotels in Swansea area such as Tawe, which was the biggest client of Swansea Suds – a company that makes soap – closes down. Aled, the owner of that company suffers substantial losses of over  £35,000 per annum. In addition, the Ocean Salvation collides with Net Gain a few days later on its way to Portsmouth. This collision is attributed to the excessive speed of the Ocean Salvation. However, the Ocean Salvation has left the UK waters, is in Brazil for another rescue operation, and has been there since 28 February 2012. Seamorgan Salvage Solutions was consequently sued by Tony and Steve, former crewmembers of the Ocean Salvation, for unpaid wages. Seamorgan Salvage Solutions

Essay on Quantification of Proteins in Solution by...

Experiment 2 Quantification of Proteins in Solution by Spectrophotometer Lab bench# 1 Introduction: Absorption spectroscopy is a common method for finding the concentration of proteins or protein complexes in a solution. Proteins absorb light at specific wavelengths and can be defined by the equation A = log (Io/I). This equation states that an absorbance at a specific wavelength, A is equal to the log of the ratio of incident light intensity (Io), to transmitted light intensity (I). A spectrophotometer can be used quantitatively and qualitatively. A spectrophotometer is used qualitatively to obtain an absorption spectrum, which can be obtained by plotting the absorbance values, over the range of wavelengths tested for the†¦show more content†¦The concentration of protein solution ( µg/ml) calculated using equation of slopes from Fig2 Test Protein | Concentration of Protein Solution ( µg/ml) | | Lowry | Coomassie Blue | UV | BSA | 1.6 | 1.4 | 1.3 | Lysozyme | 1.6 | 1.8 | 5.9 | Hemoglobin | 1.6 | 1.4 | 3.8 | Ovalbumin | 1.6 | 1.9 | 1.5 | Gamma globulin | 1.6 | 2.5 | 2.4 | Discussion: Figure 1 shows the absorption spectrum of stock solution (6x10-5M), p-nitrophenol and 0.02M NaOH, and from the graph it can be inferred that 400nm is the wavelength of maximum absorption because absorption is noted to be the highest at this point.Show MoreRelatedThe Light Energy Absorbed By Colored Compounds1430 Words   |  6 PagespKa of PNP, and to develop standard curves for determination of unknown concentration and pH of unknown samples. Quantification of proteins is needed to determine the progress of protein purification. As the protein becomes more purified, its specific activity will increase as well. In Experiment 4.1, dilutions of PNP are prepared, and the Bradford Method was used to measure protein concentration. There are different types of chromatography, and we would be using gel filtration and affinity. ChromatographyRead MoreThe Light Energy Absorbed By Colored Compounds1429 Words   |  6 PagespKa of PNP, and to develop standard curves for determination of unknown concentration and pH of unknown samples. Quantification of proteins is needed to determine the progress of protein purification. As the protein becomes more purified, its specific activity will increase as well. In Experiment 4.1, dilutions of PNP are prepared, and the Bradford Method was used to measure protein concentration. There are different types of chromatography, and we would be using gel filtration and affinity. ChromatographyRead MoreLab Report On Chemical Tests1279 Words   |  6 Pagessulfate, carbohydrate tests to determine presence of carbohydrates and reducing sugars and, protein test to determine the contaminations present in the fucoidan. 1.5.1 Screening for Carbohydrates 1.5.1.1 Molisch’s Test 2mL of Sulfuric acid will be added to the isolate and 0.2mL alpha napthol. The formation of bluish violet zone indicated the presence of carbohydrates. 1.5.1.2 Fehling’s Test Fehling’s solution (2.5 mL each of Fehling’s AB) will be boiled in a test tube. Equal amount of the isolateRead MoreSpecific Pathogen-Free Animal Rats1525 Words   |  7 Pagesventricular myocardial tissues were collected from each group and fixed in 10% formalin, processed and embedded in paraffin wax. Thin sections of 3-5 microns thickness were cut and placed on microscopic slides. The tissues were deparaffinized in xylene solution, rehydrated in downstream serial dilutions of ethanol and stained with hematoxylin for 10 minutes, bluing for 10 minutes in running tap water, decolorized for 3 seconds by 1% acid alcohol, and the tissues were stained with eosin for 1 minute, washedRead MoreExtraction and Characterization of Proteins3660 Words   |  15 PagesCHARACTERIZATION OF PROTEINS Abstract Different techniques and principles for protein extraction and characterization were demonstrated in this experiment. Various proteins were extracted from different sources: 1.67 g yeast invertase, 1.03 g egg white albumin, and 5.15 g of milk casein. Activity assay for invertase was performed using Benedict’s test and the enzymes inverting action on sucrose was confirmed. Warburg-Christian Method and Bradford Assay were also employed to determine the protein concentrationRead MoreEssay about A Study on A. Nigra Seeds for Pharmaceutical Products1252 Words   |  6 Pagesremove the trace of remaining solvent. The extracts were subjected for vacuum drying in rotary evaporator. The free radical scavenging activity of seed extracts of A. nigra were determined using DPPH whereby solution of DPPH in 99.99% ethanol was prepared and was mixed with ethanolic solutions of n-hexane, ethyl acetate and methanol extracts from A. nigra seeds at determined concentrations. The mixture was shaken and incubated. Butylated hydroxyl toluene (BHT) as positive reference ethanol as negativeRead MoreSex Determination By Amplification Of Amelogenin Gene From Dental Pulp Tissue By Pcr1727 Words   |  7 Pages These are rich sources of DNA and free from contamination by external factors. During the early stages of tooth development, cells of the inner enamel epithelium, differentiate to form ameloblasts synthesizing specific proteins as enamel matrix. Amelogenin being one such protein is present in the dental pulp tissue. The presence of two Amelogenin genes was confirmed by studies of Lau et al. (1989), Shimokawa et al. (1989); one on the X-chromosome and the other on the Y-chromosome. One copy was locatedRead MorePepper Seed Dna Extraction2422 Words   |  10 Pagesgenomic DNA. The sample is lysed in DNAzol (Chomczynski et al. 1997). Both chloroform and ethanol are used to precipitate DNA. These chemicals help separate proteins and polysaccharides from nucleic acids. Because chloroform is denser then water, it will form the bottom layer after centrifuging. This lower layer is what contains the proteins and polysaccharides, leaving the DNA in the upper layer to be collected. DNA is then precipitated and washed with ethanol. TE buffer is used to solubilize theRead M oreA Brief Note On The And Dental Caries And Periodontal Diseases And Non Tobacco Users3209 Words   |  13 Pagesbacteria that acts as an agent in promoting and initializing carious lesions.11 Studies have shown that the bacterium has adhesins which allow it to bind to complementary receptors on a substrate. A. viscosus binds to salivary acidic proline-rich proteins (PRPs) adsorbed onto the apatitic surfaces of the tooth. Cryptic segments exposed in adsorbed molecules are identified by the adhesins, which allow A. viscosus to efficiently attach to teeth while suspended in saliva.12 Actinomyces naeslundii Actinomyces

Business Plan for Sandwich Shop free essay sample

Where do you operate ? What is the company size ? For example, we are a small restaurant in Miami and we are preparing these kind of foods, or Mexican specific. 2) Company Background [Information about your company, also is good to specify your website here. ] The kind of services you provide. You have been successful in the following field and have a list of accomplishments. 3) Current Operations and processes [Describe here the current process of your company so the customer will know with what you are dealing in the present time] You currently serves 4) Market Analysis [Make here an analysis of your costs and profits estimates. For example put a graphic for it] Also target your market. For example as a restaurant you will target people who live in the restaurant neighborhood and inside driving distance. Also describe what kind of people will buy a product. How old they are ? What gender they are ? What occupation they have ? Where do they live ? 5) Service offered [Make a description of the service you are offering or the kind of business relationship you are proposing] For example here you can make a list of your products : ?product name ?product description ?product image Explain how the customer will benefit from your services. We will write a custom essay sample on Business Plan for Sandwich Shop or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page 6) Management describe in detail how you will accomplish the clients needs and make results with your products] Put also here the phases of your project : ?starting ?how long it will take ?the milestones of your project ?success ?payment 7) References [Name one or more companies that can make testimonials about you and your business that will put in favor as a candidate for the client] For example another client of your company which used your services and now he declare himself as a satisfied customer. 8) Contracts and Prices [Name your prices and conditions] Setup a contract between you and the customer so all the important matters will be clarified.

Research Proposal on E-Banking Essay Example

Research Proposal on E-Banking Essay E-banking or web-banking is the one of the types of the distance banking, which enables clients to take advantage of the services provided by a bank with the help of the computer under the assistance of the Internet. In order to get access to the services of E-banking one requires the standard Internet browsers Google Chrome, Internet Explorer, Opera, Mozilla, etc, so it is obvious that one does not need to install any additional software. In the modern dynamic time people do not have the opportunity to wait long hours in the bank queues waiting for their turn to get access to the services they require. Years ago, when the Internet did not exist, all the services could be received only in the bank house. Nowadays the number of the people who use the banking services has grown enormously, because in the modern society one can hardly survive without the account in a bank. So, the pressure on the bank clerks has increased seriously. The only way out to serve for millions of clients in a day time is the E-banking service which enables people solve their banking problems themselves without the need to visit the bank itself. With the help of E-banking clients are able to fulfill all possible operations: control the condition of the personal account, block the banking card if it has been lost or stolen, pay taxes, send money to another account, change currency, etc. E-banking is a positive innovation, which enables banks to serve for the greater number of clients and to improve the quality of operations. E-banking is a reasonable decision of the banks to increase the number of clients, save their time and money. If one wants to dwell on the E-banking services, he should study the issue in detail and collect information to understand the structure and function of the Internet banking is the appropriate way. A successful research proposal is supposed to contain the points important for the research, the methods used for the investigation and observation of the issue and the literature sources used for the research on E-banking. The student is expected to discuss the worthy questions about the Internet banking in order to make the research paper informative and captive. We will write a custom essay sample on Research Proposal on E-Banking specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Research Proposal on E-Banking specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Research Proposal on E-Banking specifically for you FOR ONLY $16.38 $13.9/page Hire Writer It is not easy to prepare a successful research proposal from the first attempt, because the process of writing requires logic, ability to operate facts and analyze the issue in the appropriate way. It is possible to make the process of writing less troublesome with the help of a free example research proposal on E-banking in India placed in the net. The student is able to learn about the format of the paper and the organization of the text with the help of a free sample research proposal on E-banking in Pakistan. At EssayLib.com writing service you can order a custom research proposal on E-Banking topics. Your proposal will be written from scratch. We hire top-rated PhD and Master’s writers only to provide students with professional research proposal help at affordable rates. Each customer will get a non-plagiarized paper with timely delivery. Just visit our website and fill in the order form with all proposal details: Enjoy our professional research proposal writing service!